abstract：:Leptospirosis, an emerging zoonotic disease, remains poorly understood because of a lack of genetic manipulation tools available for pathogenic leptospires. Current genetic manipulation techniques include insertion of DNA by random transposon mutagenesis and homologous recombination via suicide vectors. This study des...

journal_title：Applied and environmental microbiology

authors： Pappas CJ,Benaroudj N,Picardeau M

更新日期：2015-05-01 00:00:00

abstract：:The key enzymes and pathways involved in polyhydroxyalkanoate (PHA) biosynthesis in haloarchaea have been identified in recent years, but the haloarchaeal enzymes for PHA degradation remain unknown. In this study, a patatin-like PHA depolymerase, PhaZh1, was determined to be located on the PHA granules in the haloarch...

journal_title：Applied and environmental microbiology

authors： Liu G,Hou J,Cai S,Zhao D,Cai L,Han J,Zhou J,Xiang H

更新日期：2015-05-01 00:00:00

abstract：:Four Staphylococcus aureus-Escherichia coli shuttle vectors were constructed for gene expression and production of tagged fusion proteins. Vectors pBUS1-HC and pTSSCm have no promoter upstream of the multiple cloning site (MCS), and this allows study of genes under the control of their native promoters, and pBUS1-Pcap...

journal_title：Applied and environmental microbiology

authors： Schwendener S,Perreten V

更新日期：2015-05-01 00:00:00

abstract：:Human norovirus (NoV) is the leading cause of foodborne disease in the United States, and epidemiological studies have shown that fresh produce is one of the major vehicles for the transmission of human NoV. However, the mechanisms of norovirus contamination and persistence in fresh produce are poorly understood. The ...

journal_title：Applied and environmental microbiology

authors： DiCaprio E,Culbertson D,Li J

更新日期：2015-04-01 00:00:00

abstract：:The food-borne pathogenic bacterium Listeria is known for relatively low morbidity and high mortality rates, reaching up to 25 to 30%. Listeria is a hardy organism, and its control in foods represents a significant challenge. Many naturally occurring compounds, including the bacteriocin nisin and a number of plant ess...

journal_title：Applied and environmental microbiology

authors： Field D,Daly K,O'Connor PM,Cotter PD,Hill C,Ross RP

更新日期：2015-04-01 00:00:00

abstract：:The food-borne pathogen Vibrio parahaemolyticus has been reported as being present in New Zealand (NZ) seawaters, but there have been no reported outbreaks of food-borne infection from commercially grown NZ seafood. Our study determined the current incidence of V. parahaemolyticus in NZ oysters and Greenshell mussels ...

journal_title：Applied and environmental microbiology

authors： Cruz CD,Hedderley D,Fletcher GC

更新日期：2015-04-01 00:00:00

abstract：:The development of high-throughput methods, such as the construction of 18S rRNA gene clone or pyrosequencing libraries, has allowed evaluation of ciliate community composition in hundreds of samples from the rumen and other intestinal habitats. However, several genera of mammalian intestinal ciliates have been descri...

journal_title：Applied and environmental microbiology

authors： Kittelmann S,Devente SR,Kirk MR,Seedorf H,Dehority BA,Janssen PH

更新日期：2015-04-01 00:00:00

abstract：:Peramine is an insect-feeding deterrent produced by Epichloë species in symbiotic association with C3 grasses. The perA gene responsible for peramine synthesis encodes a two-module nonribosomal peptide synthetase. Alleles of perA are found in most Epichloë species; however, peramine is not produced by many perA-contai...

journal_title：Applied and environmental microbiology

authors： Berry D,Takach JE,Schardl CL,Charlton ND,Scott B,Young CA

更新日期：2015-04-01 00:00:00

abstract：:Application of a mycorrhizal inoculum could be one way to increase the yield of rice plants and reduce the application of fertilizer. We therefore studied arbuscular mycorrhizal fungi (AMF) in the roots of wetland rice (Oryza sativa L.) collected at the seedling, tillering, heading, and ripening stages in four paddy w...

journal_title：Applied and environmental microbiology

authors： Wang Y,Li T,Li Y,Björn LO,Rosendahl S,Olsson PA,Li S,Fu X

更新日期：2015-04-01 00:00:00

abstract：:The bacterial second messengers (p)ppGpp and bis-(3'-5')-cyclic dimeric GMP (c-di-GMP) regulate important functions, such as transcription, virulence, biofilm formation, and quorum sensing. In mycobacteria, they regulate long-term survival during starvation, pathogenicity, and dormancy. Recently, a Pseudomonas aerugin...

journal_title：Applied and environmental microbiology

authors： Gupta KR,Kasetty S,Chatterji D

更新日期：2015-04-01 00:00:00

abstract：:A repeated cross-sectional study was conducted to identify farm management, environment, weather, and landscape factors that predict the count of generic Escherichia coli on spinach at the preharvest level. E. coli was enumerated for 955 spinach samples collected on 12 farms in Texas and Colorado between 2010 and 2012...

journal_title：Applied and environmental microbiology

authors： Park S,Navratil S,Gregory A,Bauer A,Srinath I,Szonyi B,Nightingale K,Anciso J,Jun M,Han D,Lawhon S,Ivanek R

更新日期：2015-04-01 00:00:00

abstract：:Escherichia albertii is a recently described species that has been associated with gastroenteritis in humans and with healthy and ill birds. Most recently, it has been identified as the causative agent in a food-borne outbreak in Japan. The distribution and clinical importance of E. albertii are not well studied becau...

journal_title：Applied and environmental microbiology

authors： Lindsey RL,Fedorka-Cray PJ,Abley M,Turpin JB,Meinersmann RJ

更新日期：2015-03-01 00:00:00

abstract：:Genome analysis revealed the existence of a putative transcriptional regulatory system governing CO metabolism in Thermococcus onnurineus NA1, a carboxydotrophic hydrogenogenic archaeon. The regulatory system is composed of CorQ with a 4-vinyl reductase domain and CorR with a DNA-binding domain of the LysR-type transc...

journal_title：Applied and environmental microbiology

authors： Kim MS,Choi AR,Lee SH,Jung HC,Bae SS,Yang TJ,Jeon JH,Lim JK,Youn H,Kim TW,Lee HS,Kang SG

更新日期：2015-03-01 00:00:00

abstract：:The detection of Listeria monocytogenes from food is currently carried out using a double enrichment. For the ISO methodology, this double enrichment is performed using half-Fraser and Fraser broths, in which the overgrowth of L. innocua can occur in samples where both species are present. In this study, we analyzed t...

journal_title：Applied and environmental microbiology

authors： Lemaître JP,Duroux A,Pimpie R,Duez JM,Milat ML

更新日期：2015-03-01 00:00:00

abstract：:Methanol is already an important carbon feedstock in the chemical industry, but it has found only limited application in biotechnological production processes. This can be mostly attributed to the inability of most microbial platform organisms to utilize methanol as a carbon and energy source. With the aim to turn met...

journal_title：Applied and environmental microbiology

authors： Witthoff S,Schmitz K,Niedenführ S,Nöh K,Noack S,Bott M,Marienhagen J

更新日期：2015-03-01 00:00:00

abstract：:Pullulanase (EC 3.2.1.41), an important enzyme in the production of starch syrup, catalyzes the hydrolysis of α-1,6 glycosidic bonds in complex carbohydrates. A double mutant (DM; D437H/D503Y) form of Bacillus deramificans pullulanase was recently constructed to enhance the thermostability and catalytic efficiency of ...

journal_title：Applied and environmental microbiology

authors： Duan X,Wu J

更新日期：2015-03-01 00:00:00

abstract：:Comparative genomics have revealed massive horizontal gene transfer (HGT) between Mycoplasma species sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogen Mycoplasma agalactiae. To estimate the prevalence...

journal_title：Applied and environmental microbiology

authors： Tardy F,Mick V,Dordet-Frisoni E,Marenda MS,Sirand-Pugnet P,Blanchard A,Citti C

更新日期：2015-03-01 00:00:00

abstract：:Two hybrid bacteriocins, enterocin E50-52/pediocin PA-1 (EP) and pediocin PA-1/enterocin E50-52 (PE), were designed by combining the N terminus of enterocin E50-52 and the C terminus of pediocin PA-1 and by combining the C terminus of pediocin PA-1 and the N terminus of enterocin E50-52, respectively. Both hybrid bact...

journal_title：Applied and environmental microbiology

authors： Tiwari SK,Sutyak Noll K,Cavera VL,Chikindas ML

更新日期：2015-03-01 00:00:00

abstract：:Aldehydes are a class of chemicals with many industrial uses. Several aldehydes are responsible for flavors and fragrances present in plants, but aldehydes are not known to accumulate in most natural microorganisms. In many cases, microbial production of aldehydes presents an attractive alternative to extraction from ...

journal_title：Applied and environmental microbiology

authors： Kunjapur AM,Prather KL

更新日期：2015-03-01 00:00:00

abstract：:This study investigated the effect of a 30-cm covering of finished compost (FC) on survival of Escherichia coli O157:H7 and Salmonella spp. in active static and windrow composting systems. Feedstocks inoculated with E. coli O157:H7 (7.41 log CFU/g) and Salmonella (6.46 log CFU/g) were placed in biosentry tubes (7.5-cm...

journal_title：Applied and environmental microbiology

authors： Patel JR,Yossa I,Macarisin D,Millner P

更新日期：2015-03-01 00:00:00

abstract：:Infectious salmon anemia (ISA) is a serious disease of marine-farmed Atlantic salmon (Salmo salar) caused by ISA virus (ISAV), belonging to the genus Isavirus, family Orthomyxoviridae. There is an urgent need to understand the virulence factors and pathogenic mechanisms of ISAV and to develop new vaccine approaches. U...

journal_title：Applied and environmental microbiology

authors： Toro-Ascuy D,Tambley C,Beltran C,Mascayano C,Sandoval N,Olivares E,Medina RA,Spencer E,Cortez-San Martín M

更新日期：2015-02-01 00:00:00

abstract：:The hindguts of lower termites and Cryptocercus cockroaches are home to a distinct community of archaea, bacteria, and protists (primarily parabasalids and some oxymonads). Within a host species, the composition of these hindgut communities appears relatively stable, but the evolutionary and ecological factors structu...

journal_title：Applied and environmental microbiology

authors： Tai V,James ER,Nalepa CA,Scheffrahn RH,Perlman SJ,Keeling PJ

更新日期：2015-02-01 00:00:00

abstract：:Norovirus is one of the most common causes of acute viral gastroenteritis. The virus is spread via the fecal-oral route, most commonly from infected food and water, but several outbreaks have originated from contamination of surfaces with infectious virus. In this study, a close surrogate of human norovirus causing ga...

journal_title：Applied and environmental microbiology

authors： Warnes SL,Summersgill EN,Keevil CW

更新日期：2015-02-01 00:00:00

abstract：:Plant polyphenols are of great interest for drug discovery and drug development since many of these compounds have health-promoting activities as treatments against various diseases, such as diabetes, cancer, or heart diseases. However, the limited availability of polyphenols represents a major obstacle to clinical ap...

journal_title：Applied and environmental microbiology

authors： van Summeren-Wesenhagen PV,Marienhagen J

更新日期：2015-02-01 00:00:00

abstract：:Lyme disease, caused by the bacterium Borrelia burgdorferi sensu stricto, is an emerging zoonotic disease in Canada and is vectored by the blacklegged tick, Ixodes scapularis. Here we used Bayesian analyses of sequence types (STs), determined by multilocus sequence typing (MLST), to investigate the phylogeography of B...

journal_title：Applied and environmental microbiology

authors： Mechai S,Margos G,Feil EJ,Lindsay LR,Ogden NH

更新日期：2015-02-01 00:00:00

abstract：:Hepatitis B virus (HBV) is a deadly pathogen that has killed countless people worldwide. Saccharomyces cerevisiae-derived HBV vaccines based upon hepatitis B surface antigen (HBsAg) is highly effective. However, the emergence of vaccine escape mutants due to mutations on the HBsAg and polymerase genes has produced a c...

journal_title：Applied and environmental microbiology

authors： Yong CY,Yeap SK,Goh ZH,Ho KL,Omar AR,Tan WS

更新日期：2015-02-01 00:00:00

abstract：:Lowering the pH in bacterium-based succinate fermentation is considered a feasible approach to reduce total production costs. Newly isolated Enterobacter aerogenes strain AJ110637, a rapid carbon source assimilator under weakly acidic (pH 5.0) conditions, was selected as a platform for succinate production. Our previo...

journal_title：Applied and environmental microbiology

authors： Tajima Y,Yamamoto Y,Fukui K,Nishio Y,Hashiguchi K,Usuda Y,Sode K

更新日期：2015-02-01 00:00:00

abstract：:Tropomyosin receptor kinase A (TrkA), a receptor tyrosine kinase, is known to be associated with various diseases. Thus, TrkA has become a major drug-screening target for these diseases. Despite the fact that the production of recombinant proteins by prokaryotic hosts has advantages, such as fast growth and ease of ge...

journal_title：Applied and environmental microbiology

authors： Honda T,Yasuda T,Tanaka T,Hagiwara K,Arai T,Yoshino T

更新日期：2015-02-01 00:00:00

abstract：:Although the molecular surveillance network RotaNet-Italy provides useful nationwide data on rotaviruses causing severe acute gastroenteritis in children in Italy, scarce information is available on rotavirus circulation in the general Italian population, including adults with mild or asymptomatic infection. We invest...

journal_title：Applied and environmental microbiology

authors： Ruggeri FM,Bonomo P,Ianiro G,Battistone A,Delogu R,Germinario C,Chironna M,Triassi M,Campagnuolo R,Cicala A,Giammanco GM,Castiglia P,Serra C,Gaggioli A,Fiore L

更新日期：2015-01-01 00:00:00

abstract：:Myoviruses and podoviruses that infect cyanobacteria are the two major groups of marine cyanophages, but little is known of how their phylogenetic lineages are distributed in different habitats. In this study, we analyzed the phylogenetic relationships of cyanopodoviruses and cyanomyoviruses based on the existing geno...

journal_title：Applied and environmental microbiology

authors： Huang S,Zhang S,Jiao N,Chen F

更新日期：2015-01-01 00:00:00

abstract：:After the first isolation of livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) in 2003, this MRSA variant quickly became the predominant MRSA obtained from humans as part of the Dutch national MRSA surveillance. Previous studies have suggested that human-to-human transmission of LA-MRSA, compa...

journal_title：Applied and environmental microbiology

authors： Bosch T,Verkade E,van Luit M,Landman F,Kluytmans J,Schouls LM

更新日期：2015-01-01 00:00:00

abstract：:Multiple anthropogenic disturbances to bacterial diversity have been investigated in coastal ecosystems, in which temporal variability in the bacterioplankton community has been considered a ubiquitous process. However, far less is known about the temporal dynamics of a bacterioplankton community responding to polluti...

journal_title：Applied and environmental microbiology

authors： Wang K,Zhang D,Xiong J,Chen X,Zheng J,Hu C,Yang Y,Zhu J

更新日期：2015-01-01 00:00:00

abstract：:The presence of Bartonella spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern Spain, where 18.3% (17/93) of cattle were found to be positive by PCR combined with a reverse line blot (PCR/RLB), whereas sheep (n = 133) or horses (n = 91) were found not to be infecte...

journal_title：Applied and environmental microbiology

authors： Antequera-Gómez ML,Lozano-Almendral L,Barandika JF,González-Martín-Niño RM,Rodríguez-Moreno I,García-Pérez AL,Gil H

更新日期：2015-01-01 00:00:00

abstract：:The alphaproteobacterium Hyphomonas neptunium proliferates by a unique budding mechanism in which daughter cells emerge from the end of a stalk-like extension emanating from the mother cell body. Studies of this species so far have been hampered by the lack of a genetic system and of molecular tools allowing the regul...

journal_title：Applied and environmental microbiology

authors： Jung A,Eisheuer S,Cserti E,Leicht O,Strobel W,Möll A,Schlimpert S,Kühn J,Thanbichler M

更新日期：2015-01-01 00:00:00

abstract：:Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. The molecular basis of this inability remains unknown. We found that cells capable of assimilating mannitol arose spontaneously from wild-type S. cerevisiae during prolonged culture in ma...

journal_title：Applied and environmental microbiology

authors： Chujo M,Yoshida S,Ota A,Murata K,Kawai S

更新日期：2015-01-01 00:00:00

abstract：:A UDP glucosyltransferase from Bacillus licheniformis was overexpressed, purified, and incubated with nucleotide diphosphate (NDP) d- and l-sugars to produce glucose, galactose, 2-deoxyglucose, viosamine, rhamnose, and fucose sugar-conjugated resveratrol glycosides. Significantly higher (90%) bioconversion of resverat...

journal_title：Applied and environmental microbiology

authors： Pandey RP,Parajuli P,Shin JY,Lee J,Lee S,Hong YS,Park YI,Kim JS,Sohng JK

更新日期：2014-12-01 00:00:00

abstract：:Studies of newly emerged Apis mellifera worker bees have demonstrated that their guts are colonized by a consistent core microbiota within several days of eclosure. We conducted experiments aimed at illuminating the transmission routes and spatiotemporal colonization dynamics of this microbiota. Experimental groups of...

journal_title：Applied and environmental microbiology

authors： Powell JE,Martinson VG,Urban-Mead K,Moran NA

更新日期：2014-12-01 00:00:00

abstract：:To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sa...

journal_title：Applied and environmental microbiology

authors： Ishii S,Kitamura G,Segawa T,Kobayashi A,Miura T,Sano D,Okabe S

更新日期：2014-12-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:The model organism for polyhydroxybutyrate (PHB) biosynthesis, Ralstonia eutropha H16, possesses multiple isoenzymes of granules coating phasins as well as of PHB depolymerases, which degrade accumulated PHB under conditions of carbon limitation. In this study, recombinant Escherichia coli BL21(DE3) strains were used ...

journal_title：Applied and environmental microbiology

authors： Eggers J,Steinbüchel A

更新日期：2014-12-01 00:00:00